Research Acknowledgement

It is extremely important that our grant is cited in all publications resulting from the use of NORC services. Our productivity and effectiveness as a Center is measured in part by the citation of the grant in published work. Please use the following acknowledgement in your publications:

This work utilized Core Services supported by grant DK089503 of NIH to the University of Michigan.

Core Services: Molecular Phenotyping: Lipidomics Lab

This new laboratory provides interested researchers an opportunity to quantify and identify lipids and other biological material in cells, organs or other biological fluids of test animals or in human subjects. The laboratory works with individual investigators to develop directed methods for metabolite analysis. The analytic platforms include mass spectrometry instrumentation in several configurations (GC/MS, LC/MS, MS/MS) and NMR-based mass isotopomer analysis for measurement of flux in specific metabolic pathways.

A signed service agreement and hard copy of purchase order must be obtained from each outside customer. Sample service agreement.

Rates

The Molecular Phenotyping Core of the Nutrition Obesity Research Center is offering the following at the indicated cost per sample. Prices apply to studies billed directly to a NORC member utilizing a University of Michigan internal account.

Lipidomics Procedure Rates
  • Extraction of total lipids from biological samples: $76.38 per single sample
  • Separation of neutral & phospholipids, their individual lipid classes & extraction: $73.13 for 1 to 10 samples
Analysis of fatty acid composition by gas chromatography (GC)
  • Preparation of fatty acid methyl ester & purification by thin-layer chromatography: $69.32 for 1 to 5 samples
  • Analysis of fatty acid compositions by GC: $48.96 for 1 to 5 samples
Analysis of free fatty acids from plasma samples (purification followed by GC)
  • Per sample: $103.00
  • Per sample for tissue samples: $162.14
Analysis of positional distribution of fatty acids in lipids (PC, PE, PA, and DG)
  • Enzymatic reactions, separation of lipid products and their isolation: $203.99 for 1 to 2 samples
  • Preparation of fatty acid methyl ester & purification by thin-layer chromatography: $69.32 for 1 to 5 samples
  • Analysis of fatty acid compositions by GC: $48.96 for 1 to 5 samples
  • Quantization of phospholipids by phosphorus assay: $72.68 for 1 to 5 samples
  • Microscale synthesis of LPA & PA with selective fatty acyl chain: $581.40 per 1 to 2 samples
  • Unknown metabolite analysis, minimum 2 hr consult: $114.28 per consult unit

For larger sample batches, please contact us regarding bulk analysis rates.

Contact

U of M users: Please contact Steve Brown at stephecb@umich.edu or call 734-232-0842 to schedule these services. We request that a completed sample submission form be submitted with all samples (email or hardcopy are both acceptable).

External users: Please call or email for pricing schedule. 30% overhead must be included for all external users. A signed service agreement and hard copy of purchase order must be obtained from each outside customer.

Download sample service agreement (PDF).

For more information, please email Steve Brown at stephecb@umich.edu or call 734-232-0842.