Research Acknowledgement

It is extremely important that our grant is cited in all publications resulting from the use of NORC services. Our productivity and effectiveness as a Center is measured in part by the citation of the grant in published work. Please use the following acknowledgement in your publications:

This work utilized Core Services supported by grant DK089503 of NIH to the University of Michigan.

Core Services: Molecular Phenotyping: Undirected Metabolomics Lab

About the Undirected Metabolomics Lab

The Undirected Metabolomics Lab is firmly committed to the development of the science of Metabolomics, and discovery of advanced metabolomic techniques. In order to accomplish this goal, we freely collaborate with researchers from all over the world to acquire a diverse collection of sample types and experimental designs.

The Undirected Metabolomics Facility platform is based on four interwoven and fully integrated aspects:

Hardware: Because of its high sensitivity and the specificity of high-resolution mass accuracy, our platforms are Mass spectrally-based. The current generation of mass specs and the software that control them have become so powerful that it is likely that mass spectrally-based platforms will be the workhorses of the science for the foreseeable future, and can solve the major problems. None-the-less, NMR, FTIR, and other physical measurement systems clearly add benefit where mass spec is not sufficient.

Informatics: The science of Metabolomics is far more driven by the required informatics infrastructure than by the actual hardware platform. The informatics aspect begins with a LIMS system capable of tracking samples, their process through the analysis, and the results of their analysis.

Robotics: Humans are too good at thinking and being creative to do the repetitive mundane, and error prone tasks involved in most medium through-put sample handling. By automating processes wherever possible, we are able to reduce error and, thereby, enhance data quality.

Standards-based QA/QC: The use of standards, embedded into all samples and blanks, to continuously monitor all aspects of the sample preparation and analytical processes assures that the overall process may be continually improved. In addition, the ability to always know the level of error in any data set it is possible to design better experiments. Better experimental design yields more better quality information is obtainable from fewer samples.

Platforms

The Metabolomics platforms designed by Metabolic Analyses follow the dictum of parallel analyses using a variety of detectors and a variety of separation systems. Mass spectrometry, given its high sensitivity, and the richness of physical detail afforded, has always been the primary means of making these measurements. Our platform design has therefore favored a combination of LC/MS, GC/MS, or CE/MS.

Rates

The Molecular Phenotyping Core of the Nutrition Obesity Research Center is offering the following at the indicated cost per sample. Prices apply to studies billed directly to a NORC member utilizing a University of Michigan internal account.

  • Sample preparation - liquid (plasma, serum, urine): $163.95 per sample
  • Sample preparation - non-liquid (cells, tissues): $218.58 per sample
  • Develop new assay (method development): $118.36 per sample
  • LC-MS sample analysis (positive and/or negative mode): $67.37 per sample
  • GC-MS sample analysis: $67.37 per sample
  • Data processing/presentation: $63.73 per sample

For larger sample batches, please contact us regarding bulk analysis rates.

Forms

Contact

U of M users: Please contact Steve Brown at stephecb@umich.edu or call 734-232-0842 to schedule these services. We request that a completed sample submission form be submitted with all samples (email or hardcopy are both acceptable).

External users: Please call or email for pricing schedule. 30% overhead must be included for all external users. A signed service agreement and hard copy of purchase order must be obtained from each outside customer.

Download sample service agreement (PDF).

For more information, please email Steve Brown at stephecb@umich.edu or call 734-232-0842.